Food- and activity-related establishments are increasingly viewed as neighbourhood resources that potentially condition health-related behaviour. The objective of the current study was to establish, using on-site verification, the validity of measures of availability of food stores and physical activity establishments that were obtained from commercial database and Internet searches. Such commercial data sources may provide a valid alternative to field observations and could prove a valuable tool in the evaluation of commercial environments relevant to eating behaviour. In contrast, this study did not find strong evidence in support of commercial and Internet data sources to represent neighbourhood opportunities for active lifestyle.