This report provides guidance to Federal land managers on how to promote bicycling. Bicycling facilities are important transportation and recreation links to connect gateway communities, visitor centers, campgrounds, trailheads, and other attractions on Federal lands. This report presents benefits of bicycling, successful bicycling programs, policies that support bicycling, issues and challenges faced by land managers, and useful resources available to help meet these challenges. Bicycle transportation networks have significant positive impacts for the environment, health and visitor experience on Federal lands. Federal land managers have the opportunity to serve as positive national role models by mainstreaming bicycling to create sustainable transportation networks.